| Region name | Forward primer | Reverse primer |
|---|---|---|
| D.melanogaster DGO | TGGCCTGTGCCATGTGTGCGAGTACG | TGGGTGCGCAATTATGCCGCCAGAGC |
| D.melanogaster DG1 | CTGGGTGTGTGTGCAATATGTGAGC | GTGAGGGTACAAGGCGAAATCGAAA |
| D.melanogaster DG2 | AATTGTTCGCACGCTTCGCTCTAA | GATTGGTGCCGAGAGGTGAAAGTG |
| D.melanogaster DG3 | GGCCACAACTCAATGGCAAAAATG | CAGCAGCGAATCAAGACGAAAGGT |
| D.melanogaster DG4 | CCCCCGTCTTTGTCTGTTTGTCTG | GGAACACAATCTGCCTGCCTGACT |
| D.melanogaster DG5 | TATCCTTTTACGACGCCCCTGTGTC | GATTCGGTTCCTTGGGATTGGATTT |
| D.melanogaster Z | ATTGCTTCGGCTCTCCCGTTA | TTGTGTGGCTCACTTGGCAC |
| D.simulans Zprox | GTGAAAGATCGGATCCGTCT | GTTCGTATCGCCCACTTGAAT |
| D.simulans Z | ATTGCTTCGGCTCTCCCGTTA | TTATGTGGCTCACTTGGCAC |
| D.sechellia Z | ATTGCTTCGGCTCTCCCGTTA | TTGTGTGGCTCACTTGGCAC |
| D.simulans DG2 | TGCTTTTCCAACCCCTCAGTT | GGGGGTGCAGGCTATTTTGTTC |
| D.sechellia DG2 | TGCTTTTCCAACCCCTCAGTT | GAGGGTGCAGGCTATTTTGTTC |
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