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. Author manuscript; available in PMC: 2010 Jul 24.
Published in final edited form as: Nat Chem Biol. 2007 Jun 17;3(7):423–431. doi: 10.1038/nchembio.2007.4

Figure 5.

Figure 5

Simulation of CaGF signals. All panels show results after 20 ms of depolarization for 7 pS calcium channels with 100 mV driving force. Channels or clusters of channels are assumed to be uniformly distributed. (a) CaGF activated by an isolated open channel. Shown is the predicted fractional activation of CaGF as a function of lateral distance from the channel (red squares, 1 mM BAPTA; black circles, 40 mM EGTA; blue triangles, 4.5 mM EGTA; gray inverted triangles, small-molecule buffers absent) and a hypothetical dye with 100-fold higher affinity (1 mM BAPTA, magenta diamonds; no buffer, cyan circles). (b) Fraction of bound CaGF (thin lines, open symbols) and depletion of buffers (thick line, filled symbols) around a cluster of 64 channels; buffers as in a. (c) Free calcium around a cluster of 64 channels. (d) Effect of cluster size. Filled symbols, response in 1-μm hot spot. Open symbols, whole-cell response.