Figure 2.

Elongated H60a supports efficient cell conjugate formation with NK cells. (A) Untransfected RMA cells (UN), or RMA cells expressing H60a or H60a-CD4, were labelled with PKH67 membrane dye, mixed with IL-2-expanded NK cells that had been labelled with PKH26 membrane dye and conjugate formation was then investigated using flow cytometry, as described in the Materials and methods. NK cells were pooled from groups of five mice. (B) Graph showing data from three independent experiments (1, 2 and 3), using different batches of NK cells. Experiment 1 is shown in (A).