Figure 6. Numb function in adherens junctions and NFP^EKO epicardial phenotype.

(A) Numb localized to adherens junctions in control but not in βcat^EKO epicardial cells. Arrowheads indicate adherens junctions. (B) Numb, Par3, N-cadherin (N-cad) and β-catenin (β-cat) were immunoprecipitated from primary epicardial cell lysates and blotted for the indicated proteins. (C) N-cadherin was immunoprecipitated from control and βcat^EKO epicardial cell lysates and blotted for interactions with the indicated proteins. Input was 5% of the lysate. (D) Fewer WT1⁺ cells were present in the myocardium of NFP^EKO than in control hearts. (E) Ad- GFP was used to trace epicardial cell EMT in an ex vivo culture system. (F) Adherens junctions (α-catenin and N-cadherin) and tight junctions (ZO-1) in NFP^EKO epicardial cells. Arrowheads point to junctions. Podoplanin was used to identify epicardial cells. Scale bar = 10 µm. See also figure S4.