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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Exp Mol Pathol. 2009 Dec 23;88(2):225–233. doi: 10.1016/j.yexmp.2009.12.004

Fig. 1.

Fig. 1

Co-immunoprecipitation analysis of Mad2-Nek2 association. (A) 293T cells were transfected with the plasmids, as indicated. Top panel: Immunoprecipitation (IP) using anti-FLAG antibody followed by Western blot (WB) using anti-GFP antibody. Middle panel: Western blot of cell lysate using anti-GFP antibody. Bottom panel: Reprobing of the top panel blot using anti-FLAG antibody. (B) 293T cells were transfected with the plasmids, as indicated. Upper panel: IP using anti-FLAG antibody and Western blot using anti-GFP antibody. Lower panel: Western Blot of cell lysate using anti-FLAG antibody. (C) 293T cell lysate was subjected to IP using anti-Nek2 antibody (lane 3) or control mouse IgG (lane 2) followed by Western blot using anti-Mad2 antibody. The endogenous Mad2 level in the cell lysate was also examined (lane 1). (D) Diagram of Nek2A and its deletion mutants. LZ: leucine zipper; CC: coiled-coil domain.