Table 2.
Primer used to amplify the dopamine receptor, beta-actin and their competitive mutants
product | |||
Target | Primer | primer sequence | length (bp) |
D3R | Upstream | 5'-ACG-ACA-TGG-CTG-GGC-TAC-G-3' | 155 |
Downstream | 5' -GCC-AAC-AGC-CTG-GCG-CTA-G-3' | ||
D5R | Upstream | 5'-GTC-GCC-GAG-GTG-GCC-GGT-TAC-3' | 362 |
Downstream | 5'-GCT-GGA-GTC-AGA-ATT-CTC-TGC-AT-3' | ||
BA | Upstream | 5'-CGT-GGG-CCG-CCC-TAG-GCA-CCA-3' | 260 |
Downstream | 5'-TTG-GCC-TTA-GGG-TTC-AGG-GGG-G-3' | ||
Mutant D3R | Upstream | 5' -TCA-AGA-GTT-CCC-TAT-CAC-TCT-CAA-GTT- | 474 |
TCG-TGA-GCT-GAT -TG-3' | |||
Down stream | 5'-GTC-GCC-GAG-GTG-GCC-GGT-TAC-CAA-GTT- | ||
TCG-TGA-GCT-GAT -TG-3' | |||
Mutant D5R | Upstream | 5'-GTC-GCC-GAG-GTG-GCC-GGT-TAC-CAA-GTT- | 435 |
TCG-TGA-GCT-GAT -TG-3' | |||
Downstream | 5'-GCT-GGA-GTC-AGA-ATT-CTC-TGC-ATA-TTT- | ||
GAT-TCT-GGA-CCA-TGG-C-3' | |||
Mutant βA | Upstream | 5'-TTG-GCC-TTA-GGG-TTC-AGG-GGG-GAT-TTG- | 429 |
ATT-CTG-GAC-CAT-GGC-3' | |||
Downstream | 5'-CGT-GGG-CCG-CCC-TAG-GCA-CCA-CAA-GTT- | ||
TCG-TGA-GCT -GAT-TG-3' |