Figure 2. Representative immunocytochemistry showing JunD translocated into LNCaP cell nuclei after androgen treatment.

LNCaP cells were untreated (− R1881) (A) or treated with androgen (1 nM R1881) (B) for 72h, then fixed, stained with JunD primary-AlexaFluor594 secondary antibody pair, and observed using an Olympus microscope model Mercury 100 fluorescence. (100x-magnification). Nuclei were identified by DAPI staining (data not shown). The experiment was repeated 2 times, including 6 slides in 2 replicates per condition with similar results.