Skip to main content
. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Cancer Res. 2010 May 11;70(11):4560–4568. doi: 10.1158/0008-5472.CAN-09-3596

Figure 4. Androgen-induced increase in SSAT promoter activity is abrogated when JunD is silenced in LNCaP cells.

Figure 4

A. LNCaP cells in which JunD is silenced (siJunD) and respective vector control LNCaP cells were transiently transfected with an SSAT promoter luciferase reporter vector, then treated with androgen (1nM R1881) (gray bars) or left untreated (Control) (black bars). Cell lysates were collected after 72h and firefly luciferase activity was measured. Data and error bars are respectively the mean and standard deviation from 18 data points of measured relative light units (RLU) normalized to protein concentration from 6 independent repeat transfection/treatment experiments (n=3 samples per condition for each experiment). P-values were calculated using a two-tailed Student’s t-test assuming unequal variance. B. SSAT mRNA levels as determined by qRT-PCR showing a >25-fold increase in SSAT mRNA in vector control cells and only 10-fold increase in SSAT mRNA in JunD silenced cells (siJunD). Results are presented as a ratio of mRNA in androgen-treated/androgen-untreated cells after normalizing for corresponding 18rRNA. Data are mean of three independent observations.