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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Cancer Res. 2010 May 11;70(11):4560–4568. doi: 10.1158/0008-5472.CAN-09-3596

Figure 5. ChIP assay identifying a binding site for JunD but not AR within the SSAT promoter sequence.

Figure 5

ChIP assay studies were carried out in LNCaP cells treated with androgen (1nM R1881) using primer pairs targeted to identify the SSAT promoter sequence (see text). A. Agarose gel electrophoresis of PCR products showing the only PCR product obtained, which was from DNA fragments immunoprecipitated by JunD antibody (Lane:JunD) using the F1R1 primer pair. Using the same F1R1 primer pair, no PCR product was obtained from immunoprecipitation of chromatin fragments by AR antibody (Lane:AR), nor from the non-specific IgG (Lane:IgG) controls. M: DNA Ladder size marker. B. Sequence of the PCR product, which was cloned into PCR2.1TOPO and sequenced using M13 primer, matches −574 to −651bp of the SSAT gene promoter (NCBI accession#1103903).