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. Author manuscript; available in PMC: 2011 Sep 15.
Published in final edited form as: J Comp Neurol. 2010 Sep 15;518(18):3803–3818. doi: 10.1002/cne.22425

Figure 5.

Figure 5

CALM is present in human embryonic stem cells, whereas AP180 is expressed only in neurons differentiated from the embryonic stem cells. A: AP180 mRNA was undetectable by RT-PCR in undifferentiated (Und) NTear2 cells and cells differentiated in culture for one week (1week), but became detectable in cells differentiated for two or three weeks. B-D: The undifferentiated NTear2 cells stained positively for embryonic stem cell markers (red), Nanog, Oct3/4, and SSEA4. None of these cells showed AP180 staining (green). Scale bar: 20 μm. E: Unlike AP180, CALM mRNA was expressed in undifferentiated and differentiated NT2 cells. F-H: Double immunostaining of CALM (green) and embryonic stem cell markers (red) in undifferentiated NTear2 cells. Scale bar: 20 μm.