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. Author manuscript; available in PMC: 2010 Jul 26.
Published in final edited form as: J Investig Dermatol Symp Proc. 2009 Aug;14(1):20–24. doi: 10.1038/jidsymp.2009.8

Figure 1.

Figure 1

Basal gene expression of MMP family members in human skin in vivo. Full thickness (4mm) sun-protected buttock human skin was obtained from healthy adult human volunteers (eight subjects, average 36 years age), as previously described (Fisher, G.J, 1991. J Invest Dermatol 96:699–707; Quan, 2004, Amer J Pathol 169:482–490). Total RNA was extracted, using a commercial kit (RNeasy midikit, Qiagen, Chatsworth, CA) according to the manufacturer’s protocol. Reverse transcription was performed using Taqman Reverse Transcription kit (Applied Biosystems, Foster City, CA). Real-time RT-PCR was performed using a Taqman Universal PCR Master Mix kit (Applied Biosystems, Foster City, CA) and 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA). All primers and probes were purchased from Applied Biosystems (Assays-on-Demand™ Gene Expression Products). Results are means±SEM of MMPs mRNA normalized to 36B4 (internal control) mRNA. All procedures involving human subjects were conducted in accord with the regulations set forth by the University of Michigan Institutional Review Board, and all subjects provided written informed consent.