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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Cancer Res. 2010 Jun 1;70(13):5358–5367. doi: 10.1158/0008-5472.CAN-10-0027

Figure 1.

Figure 1

Taspase1 is Required for the Maintenance of MYC-RAS Transformed Mouse Embryonic Fibroblasts (MEFs). A, MYC-RAS transduced MEFs of the indicated genotypes were treated with 500 nM 4-hydroxytamoxifen (4-OHT) for 6 hours to activate R26-creERT. Deletion of the conditional (floxed; f) Taspase1 allele was confirmed by PCR at day 3. B, 105 MYC-RAS transduced MEFs of the indicated genotypes were mock (DMSO) or 4-OHT treated for 6 hours before plating on 6 cm dishes. Cells were photographed and counted at day 5. Scale bar equals 100 μm. Data presented are mean ± SD of duplicates of three independent experiments. C, 5×104 MYC-RAS transduced MEFs of the indicated genotypes were treated as B and plated on soft agar. Positive clones (>200 μm) were scored 1.5-2 weeks after the initial plating. Insets are higher-magnification images. Scale bar equals 500 μm. Data presented are mean ± SD of duplicates of two independent experiments. The average of colony numbers in mock treated plates was assigned as 1. Asterisk indicates P < 0.0005, determined by Fisher’s exact test.