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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Cancer Res. 2010 Jun 1;70(13):5358–5367. doi: 10.1158/0008-5472.CAN-10-0027

Figure 3.

Figure 3

Taspase1 is Required for the Proliferation of Human Cancer Cells. A, The protein levels of Taspase1 and β-actin of the indicated human cancer cell lines were assessed by anti-Taspase1 α28 and anti-β-actin antibodies. The relative expression of Taspase1 versus β-actin in hTERT-BJ-1 cells was assigned as 1. B, Human cancer cell lines with control- or Taspase1-knockdown were plated on 6-well plates and cell numbers were counted four days after the initial plating. The average cell number of control-knockdown cells was assigned as 1. Data presented are mean ± SD of duplicates of three independent experiments. C, The stable knockdown of Taspase1 in the indicated human cancer cell lines was determined by anti-Taspase1 α28 immunoblots. The expression of p16, p21, p27, Cyclin E2, and Cyclin A was determined by respective antibodies. The anti-β-actin immunoblots indicate equal protein loading.