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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Cancer Res. 2010 Jun 1;70(13):5358–5367. doi: 10.1158/0008-5472.CAN-10-0027

Figure 4.

Figure 4

Deficiency of Taspase1 Results in Increased Anoikis and Decreased Anchorage-independent Growth of SK-MEL-2 Melanoma and U251 Glioblastoma Cells. A, Human cancer cell lines with control- or Taspase1-knockdown were stained with annexin V and analyzed by FACS. B, Human cancer cell lines with control- or Taspase1-knockdown were cultured in PolyHEMA pre-treated 6-well plates for 3 days, stained with annexin V, and analyzed by FACS. C, SK-MEL-2, U251, or HT-29 cells with control- or Taspase1-knockdown were plated on soft agar. Positive clones (>100 μm) were scored 2-3 weeks after the initial plating of 105 cells. Scale bar equals 500 μm. Annexin V stains positive for apoptotic cells. Data presented in A, B, and C are mean ± SD duplicates of three independent experiments.