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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Cell. 2010 May 28;141(5):859–871. doi: 10.1016/j.cell.2010.03.053

Figure 2.

Figure 2

Overexpression of Bcl-xL and XIAP constructs blocks LTD in CA1 neurons of organotypic hippocampal slice cultures. CA1 neurons were biolistically transfected with Bcl-xL, XIAP-Bir1,2, XIAP-Bir3 or CrmA (with GFP cotransfected as a visual marker) and recorded in whole-cell patch-clamp mode. (A–C) Pairwise analysis of the effects of Bcl-xL (11 pairs of transfected and neighboring untransfected cells), XIAP-Bir1,2 (11 pairs) or CrmA transfection (10 pairs) on basal AMPA-EPSC amplitude (left graph, recorded at a holding potential of −70mV) and NMDA-EPSC amplitude (right graph, recorded at a holding potential of +40mV) versus nearby untransfected cells (pairs of transfected and neighboring untransfected cells are individually plotted; black circles). Red symbol and error bars indicate mean ± SEM. Bcl-xL and XIAP caused an increased basal EPSCAMPA (rightward shift on graph) but had no effect on the EPSCNMDA, in comparison with neighboring untransfected cells. (D) Induction of LTD was blocked in CA1 cells overexpressing XIAP-Bir1,2 (p>0.05, n=7), and BclxL (p>0.05, n=8). LTD was intact in non-transfected CA1 neurons from organotypic hippocampal culture (p<0.01, n=6). (E) LTD induction was blocked by overexpression of XIAP-Bir3 (n=8) but not CrmA (n=8). (F) LTP was unaffected by overexpression of Bcl-xL (n=7), XIAP-Bir1,2 (n=7) or XIAP-Bir3 proteins (n=7). Graphs show mean ± SEM.