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NKX2-5 is ectopically induced in skeletal muscle. (a) Transgene expression in induced mice. DAPI staining marks nuclei; GFP expression indicates transgene expression. Immunohistochemistry for NKX2-5 (red) shows that there is no expression in uninduced skeletal myonuclei but markedly increased expression in muscle from induced mice. (b) Protein blotting for NKX2-5 in skeletal muscle. GAPDH was used as a loading control.
