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. Author manuscript; available in PMC: 2010 Jul 26.
Published in final edited form as: Nat Genet. 2007 Dec 16;40(1):61–68. doi: 10.1038/ng.2007.28

Figure 6.

Figure 6

NKX2-5 is expressed in muscle tissue from individuals with DM1. (a) DAPI staining for myonuclei and immunohistochemistry for NKX2-5 in skeletal muscle from individuals with or without DM1. NKX2-5 expression is present in muscle from individuals with DM1 (note green nuclear staining). (b) Protein blotting of muscle protein extracts shows NKX2-5 in tissue from individuals with DM1 (lanes 1–4) but not in normal muscle (lanes 5, 6), in muscle from individuals with Duchenne muscular dystrophy (lane 7) or in muscle from individuals with X-linked myopathy (lane 8). Lane 9: positive control (normal human heart). GAPDH was used as a loading control. NKX2-5 expression seems higher in hearts from individuals with DM1 than in hearts from individuals without DM1. (c) RT-PCR shows that expression of NKX2-5 and its downstream targets NPPA and NPPB is specific to muscles from individuals with DM1. GAPDH was used as a loading control. MD, muscular dystrophy.