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. Author manuscript; available in PMC: 2010 Jul 26.
Published in final edited form as: Nat Genet. 2007 Dec 16;40(1):61–68. doi: 10.1038/ng.2007.28

Figure 7.

Figure 7

Reversal of RNA toxicity and molecular changes. (a) CX40 expression (red) is reduced in the cardiac conduction system of induced mice but restored to more normal levels in reverted mice. Insets show differences in CX40 (red) and CX43 (green) expression in uninduced and induced ventricular myocardium. (b) Upper panel: protein blot shows that CX43 expression is restored in hearts of reverted mice. Lanes 1–3: uninduced mice; 4–6: induced mice; 7–9: reverted mice. Numbers below lanes indicate relative expression. Lower panel: RT-PCR shows that Gia1 (Cx43) mRNA expression is restored in reverted mice. (c) Reverted mice have normal Nkx2-5 expression in cardiac muscles, as shown by a count of nuclei and by RT-PCR. (d) NKX2-5 in skeletal muscle is undetectable by immunohistochemistry (upper panel) or by protein blotting (lower panel) in reverted mice. Lanes 1–2: uninduced mice; 3–5: induced mice; 6–8: reverted mice. GAPDH was used as a loading control. Graphs in b and c show mean ± s.d.