Figure 1. The calcified nodules in bovine calcifying vascular cells (CVC) accumulate not only calcium but also neutral lipids.
CVC at 90–95 % confluence was treated with osteogenic medium containing 2.0 mM phosphate. After 14 days in culture, cells were stained with A) Alizarin Red and B) Oil Red O, which identify calcium mineral and neutral lipid as red, respectively. Arrows indicate calcified nodules. C) Ca content. D) TLC analysis. E) triglycerides and F) and Cholesterol levels.