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. Author manuscript; available in PMC: 2011 Jun 25.
Published in final edited form as: Circ Res. 2010 Apr 29;106(12):1807–1817. doi: 10.1161/CIRCRESAHA.109.212969

Figure 4. FXR activation reduced phosphate-induced JNK activation.

Figure 4

A) CVCs were pretreated with INT-747 (3 μM) for 24 hours in the presence of high phosphate. Parallel immunoblots were run from same cell lysates using antibodies against the phosphorylated JNK (p-JNK), total JNK, p38 MAPK (p-p38MAPK), phosphorylated ERK (p-ERK), total ERK, phosphorylated phosphorylated AKT (p-AKT) and GAPDH. Data are representative of two experiments with similar results. B) Alizarin staining and C) Ca content. CVC were treated with SP600125 at the indicated concentration for 14 days in the presence of high-phosphate. D) ALP, COL1A1, Msx2 and osterix expression in CVC treated with SP600125 at 20 μM for 14 days. **p<0.01 vs. vehicle.