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. Author manuscript; available in PMC: 2010 Jul 26.

Published in final edited form as: Dev Dyn. 2008 Sep;237(9):2450–2459. doi: 10.1002/dvdy.21678

In Panel A, fluorescence photomicrographs of kidney cortex from P25 bcl-2 +/+, bcl-2 −/− and Hox b7/bcl-2;bcl-2 −/− mice stained with anti-PTP 1B and co-stained with Lotus tetragonobus agglutinin (to identify proximal tubules) and Dolichos biflorus agglutinin (to identify collecting ducts) in the lower panel. The arrow head points to staining on the cell periphery as observed in normal kidneys. Please note the intense PTP1B staining of distal tubules in kidney cortex from bcl-2 −/− mice. In Panel B, Western blot analysis was done on kidney protein lysate (20 μg) from P25 bcl-2 +/+, bcl-2 −/− and Hox b7/bcl-2;bcl-2 −/− mice. The membrane was incubated with antibodies to PTP 1B and reprobed with antibodies to β-catenin to control loading. Blots are representative of protein lysates obtained from 4 individual mice.

In Panel A, fluorescence photomicrographs of kidney cortex from P25 bcl-2 +/+, bcl-2 −/− and Hox b7/bcl-2;bcl-2 −/− mice stained with anti-PTP 1B and co-stained with Lotus tetragonobus agglutinin (to identify proximal tubules) and Dolichos biflorus agglutinin (to identify collecting ducts) in the lower panel. The arrow head points to staining on the cell periphery as observed in normal kidneys. Please note the intense PTP1B staining of distal tubules in kidney cortex from bcl-2 −/− mice. In Panel B, Western blot analysis was done on kidney protein lysate (20 μg) from P25 bcl-2 +/+, bcl-2 −/− and Hox b7/bcl-2;bcl-2 −/− mice. The membrane was incubated with antibodies to PTP 1B and reprobed with antibodies to β-catenin to control loading. Blots are representative of protein lysates obtained from 4 individual mice.