. Author manuscript; available in PMC: 2010 Jul 26.

Published in final edited form as: J Microbiol Methods. 2006 May 5;67(2):220–229. doi: 10.1016/j.mimet.2006.03.016

Table 1.

Primers and Probes for Taqman assays

Gene name	Accession No	Primers and Probes
M. tuberculosis IS6110^a	AF390039	1253 (+) GGGTCCAGATGGCTTGC
		1315 (-) GGGTCGCTTCCACGATG
		1275 (+) FAM-CGCGTCGAGGACCATGGAGGT-BHQ1
M. tuberculosis Hsp65^a	M15467	429 (+) GAGCTGGAGGATCCGTACG
		496 (-) TCATCGGTCTTCTTGGCTACC
		472 (-) FAM-TTGACCAGCTCGGCGCCG-BHQ1
M. avium 16S rRNA^a	M61672.1	64 (+) GAACGGAAAGGCCTCTTCG
		138 (-) GAAGTGCAGGGCAGATTGC
		88 (+) FAM-ACTCGAGTGGCGAACGGGTGAGTA-BHQ1
Human Apolipoprotein B^a	NM_000384	4253 (+) TGGCAACACCAGCACAGAC
		4326 (-) GCAGGTCAACCACAGAGTCAG
		4280 (+) FAM-CCTTCGGGCTCGTTACCACATGAA-BHQ1

Lowest limit of quantification of each assay is ten targets