Table 3.
Amplification of M. avium DNA in the presence or absence of 2.4 × 107 genomes of human DNA background following the OBL extraction method
| M. avium | ||||
|---|---|---|---|---|
| CFU submitted to DNA extraction and qPCR | Average number ± SEM of 16S rRNA targets amplified in presence (+) or absence (-) of human DNAa (sensitivity based on CFU) | Amplification efficiency in presence of human DNA (%)b | Human:MAC genome ratioc,d | |
| - | + | |||
| 270 | 206±61 (76%) | 131±77 (49%) | 64% | 31,100 |
| 2,700 | 2,105±128 (78%) | 1,453±112 (54%) | 69% | 3,500 |
| 27,000 | 53,600±330 (199%) | 39,430±377 (146%) | 74% | 190 |
| 270,000 | 441,264±16,910 (163%) | 472,860±9,259 (175%) | 107% | 30 |
a
Data shown for number of M. avium targets in the 60 μl final volume, estimated from results obtained from a 5 μl specimen submitted to qPCR
b
Percentage of M. avium targets amplified when human DNA is in background, compared to no human DNA background as reference
c
Calculations based on estimate that there is one genome for every 16S rRNA for M. avium, or ApoB gene for human
d
Data rounded to the nearest 10th