Distribution of DR markers in control (unwounded) normal and DR organ-cultured corneas. Note continuous BM staining for laminin-10 α5 chain, strong epithelial staining for α3 integrin and lack of staining for MMP-10 in normal (N) corneas. In DR corneas, little staining is seen for laminin-10, weak and disorganized staining for α3 integrin, and strong staining for MMP-10. Thus, all markers in organ-cultured corneas have the same patterns as in the respective intact normal or DR corneas. All presented corneas were kept in organ culture for 10 days. Central parts of all corneas are shown. E, epithelium; S, stroma; arrows point to the epithelial BM. Indirect immunofluorescence.