Figure 1.

Phase-contrast microscopy of primary TMC (A–F) and ONHA cells (G–M). After treatment with minocycline 20 μM and 40 μM for both cell lines (TMC: C, E; ONHA: I, L), no morphologic changes could be detected. When cells were treated additionally with 600 μM H₂O₂, neither TMC nor ONHA pretreated with minocycline 20 μM and 40 μM (TMC: D, F; ONHA: K, M) showed any significant morphologic changes. In contrast, cells that were only treated with 600 μM H₂O₂ showed marked signs of destruction and cell death (TMC: B; ONHA: H). scale (10×)

Abbreviations: TMC, trabecular meshwork cells; ONHA; optic nerve head astrocytes.