Figure 3.

Viability of primary TMC after treatment with the investigated concentrations of minocycline (black curve, squares) and additionally treated with 600 μM H₂O₂ (grey curve, triangles), measured by MTT assay. TMC treated only with minocycline concentrations up to 40 μM showed no reduction in viability compared with the control. Minocycline 50 μM (P = 0.005) and higher for primary TMC led to a dose-dependent reduction of viability. When TMC cells were treated additionally with H₂O₂, those pretreated with minocycline >7.5 μM (P < 0.001) up to 50 μM (P = 0.002) showed significantly increased viability, with a peak at 40 μM (P < 0.001) compared with the control. Concentrations of 75 μM (P = 0.01) and higher showed a significantly decreased viability. Error bars, ± SD.

Abbreviations: TMC, trabecular meshwork cells; SD, standard deviation.