Figure 7.

Survivin mRNA expression measured by quantitative RT-PCR. ONHA treated with minocycline 40 μM showed a significant increase in survivin mRNA expression compared with the control. TMC showed this increase for minocycline 20 μM and 40 μM. TGFβ-2 decreased survivin expression, and H₂O₂ treatment slightly decreased survivin expression for both cell lines. Treatment with minocycline 20 μM and 40 μM and additional treatment with TGFβ-2 increased expression of survivin compared with cells treated only with TGFβ-2. For cells treated additionally with H₂O₂, ONHA cells only showed a slight increase and TMC cells showed a more pronounced increase when pretreated with minocycline concentrations compared with cells treated with H₂O₂ only. All differences between the two minocycline concentrations and the corresponding controls are statistically significant except for the H₂O₂ group with ONHA.

Abbreviations: TMC, trabecular meshwork cells; ONHA; optic nerve head astrocytes; RT-PCR, real time polymerase chain reaction; TGFβ-2, transforming growth factor-beta-2.