Table 3. Percent GABAergic activated neurons in Control, Paradoxical sleep deprived rats or rats with hypersomnia in paradoxical sleep.
%Fos-GAD+/TotalFos-ir | |||||||
n | PSC | PSD | PSR | ||||
Tuberal Hypothalamus | |||||||
rZI | 2 | 78.0 | (76.1 ; 81.1) | 87.5 | (87.2 ; 90.0) | 92.6 | (89.8 ; 94.5) |
ZID | 2 | 70.6 | (30.9 ; 100.0) | 67.9 | (50.5 ; 86.4) | 81.2 | (67.5 ; 91.8) |
ZIV | 2 | 95.0 | (89.4 ; 100.0) | 88.6 | (83.6 ; 91.3) | 97.7 | (94.0 ; 100.0) |
SubI | 2 | 77.2 | (67.7 ; 89.9) | 77.9 | (71.9 ; 82.6) | 92.6 | (91.0 ; 93.6) |
DA | 2 | 43.2 | (39.4 ; 48.0) | 30.5 | (27.5 ; 34.9) | 45.7 | (41.3 ; 50.9) |
DM | 2 | 73.0 | (64.7 ; 75.8) | 70.7 | (69.0 ; 71.0) | 70.2 | (66.1 ; 71.3) |
Pe | 3 | 47.2 | (42.9 ; 52.1) | 66.3 | (51.5 ; 79.5) | 61.9 | (57.2 ; 64.0) |
MTu | 2 | 29.3 | (26.4 ; 39.2) | 51.4 | (34.1 ; 62.9) | 64.3 | (59.8 ; 68.4) |
tLH | 2 | 57.7 | (50.5 ;61.7) | 68.9 | (61.2 ; 81.6) | 75.8 | (73.2 ; 81.6) |
PeF | 2 | 89.4 | (66.7 ; 90.2) | 57.9 | (52.5 ; 64.2) | 80.2 | (79.2 ; 82.7) |
TC | 3 | 48.3 | (30.5 ; 66.2) | 44.4 | (30.8 ; 55.0) | 61.1 | (59.6 ; 64.0) |
VMH | 3 | 0.0 | (0.0 ; 0.5) | 3.9 | (2.1 ; 7.4) | 4.9 | (2.5 ; 7.0) |
Arc | 4 | 30.4 | (18.8 ; 45.7) | 51.7 | (40.5 ; 63.4) | 55.4 | (40.0 ; 62.9) |
Mammillary Hypothalamus | |||||||
mLH | 1 | 11.5 | (6.0 ; 16.4) | 44.4 | (25.6 ; 50.8) | 38.9 | (24.9 ; 50.8) |
PH | 1 | 20.3 | (11.2 ; 27.4) | 24.7 | (22.2 ; 26.5) | 18.1 | (11.0 ; 26.0) |
PMV | 1 | 0.0 | (0.0 ; 9.6) | 17.5 | (3.8 ; 31.9) | 8.4 | (5.0 ; 12.2) |
PMD | 1 | 0.0 | (0.0 ; 6.3) | 10.0 | (0.0 ; 27.5) | 2.5 | (0.0 ; 6.8) |
M | 2 | 0.0 | (0.0 ; 0.0) | 2.8 | (1.2 ; 4.4) | 1.6 | (1.0 ; 2.6) |
VTM | 2 | 50.0 | (0.0 ; 100.0) | 53.3 | (32.1 ; 75.0) | 38.3 | (20.0 ; 55.8) |
SuM | 2 | 2.4 | (0.0 ; 5.6) | 2.2 | (1.4 ; 3.2) | 0.9 | (0.7 ; 1.3) |
SMT | 1 | 0.0 | (0.0 ; 0.0) | 0.0 | (0.0 ; 0.0) | 0.0 | (0.0 ; 0.3) |
Percent (median (Q1; Q3)) of Fos-ir/GAD+ neurons compare to the total number of Fos-ir cells counted on a total of 6 sections at 600µm intervals after Fos immunohistochemistry combined with GAD67 mRNA in situ hybridization. Significance values are: *P<0.05 vs PSC; #P<0.05 between PSR and PSD. Abbreviations are detailed in table 1.