Skip to main content
. 2010 Jul 26;5(7):e11766. doi: 10.1371/journal.pone.0011766

Table 3. Percent GABAergic activated neurons in Control, Paradoxical sleep deprived rats or rats with hypersomnia in paradoxical sleep.

%Fos-GAD+/TotalFos-ir
n PSC PSD PSR
Tuberal Hypothalamus
rZI 2 78.0 (76.1 ; 81.1) 87.5 (87.2 ; 90.0) 92.6 (89.8 ; 94.5)
ZID 2 70.6 (30.9 ; 100.0) 67.9 (50.5 ; 86.4) 81.2 (67.5 ; 91.8)
ZIV 2 95.0 (89.4 ; 100.0) 88.6 (83.6 ; 91.3) 97.7 (94.0 ; 100.0)
SubI 2 77.2 (67.7 ; 89.9) 77.9 (71.9 ; 82.6) 92.6 (91.0 ; 93.6)
DA 2 43.2 (39.4 ; 48.0) 30.5 (27.5 ; 34.9) 45.7 (41.3 ; 50.9)
DM 2 73.0 (64.7 ; 75.8) 70.7 (69.0 ; 71.0) 70.2 (66.1 ; 71.3)
Pe 3 47.2 (42.9 ; 52.1) 66.3 (51.5 ; 79.5) 61.9 (57.2 ; 64.0)
MTu 2 29.3 (26.4 ; 39.2) 51.4 (34.1 ; 62.9) 64.3 (59.8 ; 68.4)
tLH 2 57.7 (50.5 ;61.7) 68.9 (61.2 ; 81.6) 75.8 (73.2 ; 81.6)
PeF 2 89.4 (66.7 ; 90.2) 57.9 (52.5 ; 64.2) 80.2 (79.2 ; 82.7)
TC 3 48.3 (30.5 ; 66.2) 44.4 (30.8 ; 55.0) 61.1 (59.6 ; 64.0)
VMH 3 0.0 (0.0 ; 0.5) 3.9 (2.1 ; 7.4) 4.9 (2.5 ; 7.0)
Arc 4 30.4 (18.8 ; 45.7) 51.7 (40.5 ; 63.4) 55.4 (40.0 ; 62.9)
Mammillary Hypothalamus
mLH 1 11.5 (6.0 ; 16.4) 44.4 (25.6 ; 50.8) 38.9 (24.9 ; 50.8)
PH 1 20.3 (11.2 ; 27.4) 24.7 (22.2 ; 26.5) 18.1 (11.0 ; 26.0)
PMV 1 0.0 (0.0 ; 9.6) 17.5 (3.8 ; 31.9) 8.4 (5.0 ; 12.2)
PMD 1 0.0 (0.0 ; 6.3) 10.0 (0.0 ; 27.5) 2.5 (0.0 ; 6.8)
M 2 0.0 (0.0 ; 0.0) 2.8 (1.2 ; 4.4) 1.6 (1.0 ; 2.6)
VTM 2 50.0 (0.0 ; 100.0) 53.3 (32.1 ; 75.0) 38.3 (20.0 ; 55.8)
SuM 2 2.4 (0.0 ; 5.6) 2.2 (1.4 ; 3.2) 0.9 (0.7 ; 1.3)
SMT 1 0.0 (0.0 ; 0.0) 0.0 (0.0 ; 0.0) 0.0 (0.0 ; 0.3)

Percent (median (Q1; Q3)) of Fos-ir/GAD+ neurons compare to the total number of Fos-ir cells counted on a total of 6 sections at 600µm intervals after Fos immunohistochemistry combined with GAD67 mRNA in situ hybridization. Significance values are: *P<0.05 vs PSC; #P<0.05 between PSR and PSD. Abbreviations are detailed in table 1.