Figure 4.

S. cerevisiae SIN3 and RPD3 are critical for growth in media prepared with non-fermentable carbon sources. Wild type (BY4741 WT), sin3 null mutant (BY4741 sin3) (A), wild type (BY4742 WT) and rpd3 null mutant (BY4742 rpd3) (B) cells were grown in 5 ml of YPD, washed twice with water, inoculated into media containing glucose (YPD), ethanol (YPE) and glycerol and ethanol (YPGE) as sole carbon sources and incubated at 30°C. Absorbance at 550 nm was measured at the indicated times. Representative results of single growth curve assays are shown. A minimum of three independent trials was performed and all produced similar results.