Figure 2. Silymarin inhibits JFH-1 polymerase at high dose.
HCV NS5B polymerase from isolate JFH-1 (genotype 2a) was incubated with the indicated concentrations of Silymarin or Silibinin, respectively, in presence of [32P]GTP and polyC. Incorporation of radioactivity was quantified by TCA precipitation and liquid scintillation counting. Silymarin and to a lesser degree, silibinin, inhibited JFH-1 polymerase.