Figure 1.

Assessment of IPvTotal plots. (a) Scatterplot of immunoprecipitated (Purkinje cells, IP) versus unbound RNA (from whole cerebellum, Total) provides a basic measure of experiment quality. RNA for non-Purkinje cell genes (glial genes, red) are highly enriched in Total RNA, while RNAs determined to be in Purkinje cells (blue, Supplementary Table S1) are enriched in IP RNA. (b) Illustration of the interpretation of IPvTotal plots based on the locations of positive and negative control genes.