Figure 5.

Methylation of DNA on modified oligonucleosomes. (A) Methylation of the first three CpG sites by full-length Dnmt3a2, full-length Dnmt3a2/Dnmt3L, but not Dnmt3a-C is stimulated by the absence of H3K4me3 (data taken from Figures 3 and 4). For each comparison, the methylation levels were normalized to H3K4me3, which showed the lowest methylation. Results shown are the average of three independent experiments; error bars indicate the standard deviations. (B) Schematic picture showing how the H3 tail interaction of Dnmt3a’s ADD domain (orange) anchors the catalytic domain (red) to methylate the linker DNA regions of nucleosomes.