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. 2010 Mar 24;38(13):4285–4295. doi: 10.1093/nar/gkq170

Figure 2.

Figure 2.

Plasmid survival and reporter gene expression in HeLa cells. The cells were transfected with 400 ng pDsRed plasmid (non-damaged reference) together with the indicated amounts of pZA plasmids (non-damaged or damaged, as specified). (A) Relative GFP fluorescence as a function of the amounts of non-damaged pZA plasmid used for transfection. Means of three independent experiments (±SD). (B) Comparison of DNA amounts recovered from the cells (open columns) and GFP protein expression (black columns) for the range of transfection inputs of the non-damaged (oG = 0) and damaged (oG = 3) pZA plasmid. All values are expressed relative to the values obtained after transfection with 400 ng of undamaged pZA plasmid. (C) Messenger RNA expression 24 h after transfections with non-damaged (oG = 0) and damaged (oG = 3) pZA plasmids. cDNA copy numbers (mean and data range) were determined by real-time PCR relative to the copy numbers of DNA recovered from the transfected cells. Mean GAPDH cDNA copy number is used for normalisation between the samples (duplicates).