Figure 4.

Chromatin features characteristic for the undamaged GFP transgene. Quantification of histones with the indicated posttranslational modifications at the transiently transfected GFP gene (promoter, 3′UTR) and the promoter-proximal regions of three chromosomal genes (GAPDH, MYOD1 and AFM), and occupancy of the indicated gene fragments by RNA polymerase II (Pol2). Data of representative ChIP experiments with typical proportions in recoveries of the analysed DNA fragments (mean and data range of two determinations). Dotted line shows maximal recovery with non-immune IgG observed in the corresponding experiments.