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. 2010 Mar 24;38(13):4285–4295. doi: 10.1093/nar/gkq170

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Quantitative comparison of the chromatin components in the undamaged and damaged plasmid DNA. (A) Experimental flowchart of transfection and chromatin preparation. The reference non-damaged pDsRed plasmid is introduced as an internal standard for chromatin immunoprecipitation. (B) Summary of DNA fragments analysed by real-time PCR. (C) DNA fragment size analyses after the reversal of cross-links in the samples transfected with an undamaged pZA (lane 1) or pZA containing three oxidized guanines (lane 2). M. molecular size marker. (D) ChIP enrichments of the undamaged pZA (oG = 0, white bars) and damaged pZA (oG = 3, black bars) DNA fragments, relative to the promoter fragment of the reference pDsRed plasmid (not plotted, equals to 1 in all graphs). Error bars indicate standard deviation of the mean for n ChIP experimens.