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. 2010 Mar 24;38(13):4394–4403. doi: 10.1093/nar/gkq188

Figure 4.

Figure 4.

ΔTm of triplexes of different lengths with a G to C base mismatch at position 4. Length of pure DNA TFO or TFO with three TINAs and effect on Tm by introduction of guanine (G) to cytosine (C) base mismatch at position 4 counted from the 5′-end of the target oligonucleotide. D-200 or D-201 as target strands in combination with D-256 as AD strand and different TFO strands. The length of the TFO is counted from the 5′ to the 3′ position, and oligo IDs for TFOs used in the experiments are given below the oligo length. Oligosequences corresponding to the oligo IDs are found in Supplementary Table S4. Tm was determined using 1.0 µM of each strand in NaOAc-buffer at pH 4.5.