Table 1.

TINA in triplex TFO and change in T_m and ΔT_m

Change in triplex T_m by introduction of one to four TINAs in the TFO and change in ΔT_m by introduction of a central base mismatch or a base mismatch towards the 3′ end of the target strand. T_m was determined using 1.0 µM of each strand in NaOAc-buffer at pH 5.0. X is TINA. Base mismatches are underlined and marked in bold blue.