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TINA in triplex AD strand and change in Tm and ΔTm
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Change in triplex Tm by introduction of one or two TINAs in the AD strand and change in ΔTm by introduction of a central base mismatch or a base mismatch towards the 3′ end of the target strand. Tm was determined using 1.0 µM of each strand in NaOAc-buffer at pH 5.0. X is TINA. Base mismatches are underlined and marked in bold blue.
