Table 1.
In vitro selected RNAs, pool sequence length, pool size and motif yielda
| RNA | Sequence length (nt) | Pool size (seqs) | Yield (#/109) | Reference |
|---|---|---|---|---|
| GTP aptamer | 60–80 | 2.5 × 1014 | 0.05 | (34) |
| ATP aptamer | 169 | ∼1014 | 1–10 | (31) |
| Neomycin B aptamer | 74 | ∼1015 | 0.001 | (30) |
| Chloramphenicol aptamer | 70–80 | 1014–1015 | 0.074–0.0074 | (32) |
| Streptomycin aptamer | 74 | 1015 | 0.0043 | (33) |
| Macugen | 30 | ∼1014 | 0.0143 | (13) |
| DSL ligase | 30 | 1.4 × 1014 | 0.0001 | (35) |
| T80 ligase | 35 | ∼1014 | 0.0002 | (36) |
aMotif yield is the number of reported active RNAs per 109 sequences. This value can be biased by experimental details (e.g. RNA selection strategies, threshold values for binding constants and reaction rates).