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. 2010 Apr 28;38(13):e136. doi: 10.1093/nar/gkq299

Figure 1.

Figure 1.

Schematic of U1i. (A). When the 5′-end of endogenous U1 snRNA base pairs to a target sequence located in the 3′-terminal exon, U1 snRNP inhibits pre-mRNA polyadenylation (pA). Thus, maturation of the pre-mRNA is blocked, mRNA stability, transport to the cytoplasm, and translation are decreased and therefore gene expression is inhibited. 3′-terminal exon sequences are indicated. Intron is depicted with a dashed line. (B and C). The 5′-end of U1 snRNA has been modified to base pair Notch1 pre-mRNA resulting in U1inαNotch1. U1inαNotch1 should bind sequence CACUGCCU located four times in the Notch1 3′-UTR. Two repeats locate at positions 7924 and 8653, upstream of the first polyadenylation site. Two repeats locate at positions 10043 and 10138, upstream of an alternative polyadenylation site. An alignment of the four Notch1 target sites and neighbouring sequences is also shown.