Figure 4.

Increased inhibition is observed when U1in is combined with potent, standard and poor shRNA inhibitors. Luciferase activity was measured in HeLa cells transfected with a plasmid that expresses Firefly luciferase and a control plasmid or plasmids expressing luciferase inhibitors. 1 µg or 1/2 µg (X/2) of plasmid expressing the inhibitor was used for transfection. Inhibitors were U1inαLuc, shH2 (shαLuc158, A), shH1 (shαLuc1546, B), shS (shαLuc163, C), shL1 (shαLuc154, D) and shL2 (shαLuc230, E) alone or a combination of U1inαLuc and shαLuc (shX/2 + U1in/2). The fold inhibition is indicated for each case. Error bars are standard deviations of nine different experiments. Note that the scale is different for each figure.