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. 2010 May 5;38(13):e138. doi: 10.1093/nar/gkq333

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Full-round HHR-based RNA editing (deletion) assay based on ATPase 6 pre-edited mRNA. (A) The pre-edited ribozyme (pre-A6Rbz) is shown with the gA6Rbz gRNA that specifies the deletion of three Us from the editing site (ES) in the presence of editosome purified from mitochondrial extract (ME). The conserved (5′-CUGA-3′) of A6Rbz in the catalytic core essential for ribozyme activity is highlighted (Edited Site) and the line indicates where the three Us are removed by editing. The 3′ [³²p] pCp-label of pre-A6Rbz is indicated by an asterisk. (B) Autoradiogram showing the edited and endonuclease cleavage products (arrows) generated only in the presence of both gA6Rbz and ME. Lane T1 is pre-A6Rbz RNA subjected to partial digestion by RNase T1, which is used as marker. Percentage of RNA editing efficiency in this assay was 8.8% and it was calculated as the percentage of total input pre-A6Rbz.