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. 2010 May 7;38(13):e140. doi: 10.1093/nar/gkq354

Figure 1.

Figure 1.

Artificial miRNAs (Smart RNAs) targeting E2F1, E2F2 and E2F3. (A) Base pairing between miR-20, the three MT-E2Fs and the E2Fs binding sites. (B–D) MT-E2Fs target E2F1-3 luciferase reporters. (B) A reporter luciferase gene fused to E2F1 3′UTR. (C) A reporter luciferase gene fused to E2F2 3′UTR. (D) A reporter luciferase gene fused to E2F3 3′UTR. (E) Luciferase activity from HeLa cells co-transfected with MT-E2F(1) and E2F1 3′UTR or a mutant lacking the binding site for MT-E2F1. Paired t-test: *P < 0.05 and **P < 0.02.