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. 2010 May 7;38(13):e140. doi: 10.1093/nar/gkq354

Figure 2.

Figure 2.

MT-E2Fs target E2F1-3 endogenous protein. (A) Immunoblots on endogenous E2F1, E2F2 and E2F3 in IMR90 cells expressing the smart RNAs against E2F1-3, control hairpin and miR-20. (B) Measure of the relative miR-20 level by qPCR in the different cell lines expressing the smart RNAs against E2F1-3, control hairpin and miR-20. (C) Mcm6 immunoblots of IMR90 cells expressing the smart RNAs against E2F1-3, a control hairpin and miR-20. (D) Quantification of the immunoblots presented in (A and C) showing the relative level of E2F1, E2F2, E2F3 and MCM6 normalized on the tubulin expression for the different cell lines.