Table 2.

Pure Hex A and WT and NR forms of Hex B assayed using GM2 and a nonspecific detergent, i.e. a taurocholate:[³H]-GM2 complex, as the substrate

Form of Hex	MUG units added (nmol/h)	SA [pmol GM2 h−1 U−1 (MUG)]a
WT Hex Bb	48	0.000
NR Hex Bb	48	0.098
Pure Hex Ac	96	0.021

^aUnits (nmol/h) of MUG activity were used to calculate the specific activity.

^bHex B was isolated from CHO cells transfected with either wild-type or βAsp⁴⁵²Asn; βLeu⁴⁵³Arg encoding βcDNAs.

^cHex A purified from human placenta [17].