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Kinetic examination of purified placental Hex B using MUGS as a substrate at pH = 3.0 or 4.2. At pH = 3.0, substrate inhibition was noted with MUGS concentrations >3.5 mM (data not shown); thus assays were restricted to less than optimal substrate concentrations. The absolute specific activities (nanomoles of MU per hour per nanogram of Hex B) were plotted versus substrate concentration.
