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. Author manuscript; available in PMC: 2011 Jul 13.
Published in final edited form as: Biochemistry. 2010 Jul 13;49(27):5743–5752. doi: 10.1021/bi100618g

Figure 4.

Figure 4

A&B. Interaction of p55 and p33 proteins with HeLa cells. (A) HeLa cells were incubated with Alexa 488-labeled p55 alone (10 µg/ml) or a mixture of labeled p55 plus purified refolded p33 (5 ug/ml of each) for 4 h at 37°C degrees. Cells were imaged as described in the Experimental Procedures. (B) Cells were incubated with purified Alexa 488-labeled p55 plus purified refolded c-Myc-tagged p33 protein for 1 h at 37°C. The c-Myc-tagged p33 protein was detected by indirect immunofluorescence.