Fig. 2.

a) Hematoxylin-eosin (H&E) staining of different tissue sections in the Saline-treated (A–E) and cisplatin-treated (F–J) groups. The formation of brown hemosiderin granules were only observed in the red pulp of spleen in cisplatin-treated mice, but not in other tissues such as heart, liver, lung, and kidney, indicating the specific toxicity of low dose cisplatin in vivo. b) Representative histochemical measurements of spleen tissue sections of tumor-bearing mice in saline-treated (A–C) and cisplatin-treated (D–F) groups. The brown granules represent hemosiderin accumulation, which was mainly in the red pulp of spleen by the H&E staining (A and D). To clearly see the morphology of hemosiderin indicated by arrows, (B) and (E) are the enlarged portion of image (A) and (D), respectively. To confirm the conclusion of H&E staining, the same tissue sections were measured by the Perls’s staining (C) and (F). Scale bar =50μm.