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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Neurobiol Dis. 2010 May 20;39(3):449–456. doi: 10.1016/j.nbd.2010.05.018

Fig. 5.

Fig. 5

APP and Aβ in skeletal muscle. Equal quantities of protein (100 μg) from SDS extracted quadriceps were separated by SDS-PAGE. (A) Expression of full length APP (antibody 22C11 against the N-terminus of APP). Lysate (10 μg) from H4 neuroglioma cells over expressing human APP was run as a marker control. T7A6 mice over express human APP (~1.3x) as compared to wild type littermates. NSAID treatment did not alter the expression of APP protein. (B) Oligomeric Aβ (antibodies 6E10 + 4G8). Synthetically prepared oligomeric Aβ (3 pmol) and SDS extract from an AD case (~100 μg protein) were run as positive controls (note the appearance of monomeric Aβ slightly above the 3 kDa marker). Neither monomeric nor oligomeric Aβ was detectable in skeletal muscle from T7A6 mice.