Figure 5. Effects of antioxidant and EGF-R inhibitor on UVB-mediated PAF-R agonist production in human skin.
Human skin explants were pretreated with 50 µL of a 10 mM solution of the antioxidant vitamin C, 4 mM of the EGF-R inhibitor PD168393, or vehicle alone (10% DMSO in ethanol) for 30 min before UVB (5,000 J/m2) irradiation. The epidermis was removed at one hour post-UVB treatment and lipids extracted and tested for PAF-R agonist activity in FURA-2-AM-loaded KBP cells. Data are the mean ± SE percentage of maximal peak calcium flux induced by 1 µM CPAF and numbers of explants tested from each treatment noted in parentheses. * Denotes statistically significant (p < 0.05) differences in comparison to DMSO vehicle control.